goat polyclonal anti mouse il1b ab  (R&D Systems)

Journal: Journal of experimental zoology. Part B, Molecular and developmental evolution

Article Title: The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.

doi: 10.1002/jez.b.22733

Figure Lengend Snippet: Figure 2. Box and whisker plots representing the relative expres- sion of IL1B (A), TNF (B), and TNFRSF1A (C) in the uterus of preg- nant (n = 14) and nonpregnant (n = 5) P. entrecasteauxii. Gene expression was normalized to the geometric mean expression of ACTB and 18S. Top and bottom whisker plots represent the max- imum and minimum values, respectively. The box represents the lower and higher quartiles, and the line inside the box represents the group median. Compared to nonpregnant P. entrecasteauxii, TNF expression was 6.25-fold lower in the uterus of pregnant P. entrecasteauxii (∗F1,17 = 9.423; P < 0.01). [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: We used Western blotting to first determine whether the polyclonal goat anti-human IL1B IgG antibody (Cat.# AF-201-NA, R&D systems, Abingdon, UK) cross-reacted with P. entrecasteauxii IL1B (has 0.28% identities and 0.46% similarities with human IL1B).

Techniques: Whisker Assay, Gene Expression, Expressing

Journal: Journal of experimental zoology. Part B, Molecular and developmental evolution

Article Title: The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.

doi: 10.1002/jez.b.22733

Figure Lengend Snippet: Figure 3. Representative immunofluorescence micrograph of nonpregnant P. entrecasteauxii uterine sections (n = 3) for IL1B (green from A488 conjugated anti-goat antibodies in goat anti-human IL1B) and cell nuclei (blue from DAPI staining). Arrows = punctate staining; L = lumen; S = stroma; LE = luminal epithelium. Scale bar = 10 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: We used Western blotting to first determine whether the polyclonal goat anti-human IL1B IgG antibody (Cat.# AF-201-NA, R&D systems, Abingdon, UK) cross-reacted with P. entrecasteauxii IL1B (has 0.28% identities and 0.46% similarities with human IL1B).

Techniques: Immunofluorescence, Staining

Journal: Journal of experimental zoology. Part B, Molecular and developmental evolution

Article Title: The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.

doi: 10.1002/jez.b.22733

Figure Lengend Snippet: Figure 4. Representative immunofluorescence micrograph of stage 36 pregnant P. entrecasteauxii uterine sections (n = 3) for IL1B (green from A488 conjugated anti-goat antibodies in goat anti-human IL1B) and cell nuclei (blue from DAPI staining) indicating the placentome (A), the paraplacentome (B), and the yolk-sac uterus (C). L = lumen; S = stroma; LE = luminal epithelium. Scale bar = 10 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: We used Western blotting to first determine whether the polyclonal goat anti-human IL1B IgG antibody (Cat.# AF-201-NA, R&D systems, Abingdon, UK) cross-reacted with P. entrecasteauxii IL1B (has 0.28% identities and 0.46% similarities with human IL1B).

Techniques: Immunofluorescence, Staining

Journal: Journal of experimental zoology. Part B, Molecular and developmental evolution

Article Title: The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii.

doi: 10.1002/jez.b.22733

Figure Lengend Snippet: Figure 5. Representative immunofluorescence micrograph of stage 39 pregnant P. entrecasteauxii uterine sections (n = 3) for IL1B (green from A488 conjugated anti-goat antibodies in goat anti-human IL1B) and cell nuclei (blue from DAPI staining) indicating the placentome (A), the paraplacentome (B), and the yolk-sac uterus (C). Arrow = punctate staining; Arrow head = damaged tissue section; L = lumen; S = stroma; LE = luminal epithelium. Scale bar = 10 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: We used Western blotting to first determine whether the polyclonal goat anti-human IL1B IgG antibody (Cat.# AF-201-NA, R&D systems, Abingdon, UK) cross-reacted with P. entrecasteauxii IL1B (has 0.28% identities and 0.46% similarities with human IL1B).

Techniques: Immunofluorescence, Staining

Journal: Biology of reproduction

Article Title: Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.

doi: 10.1095/biolreprod.107.060095

Figure Lengend Snippet: FIG. 2. Immunohistochemical localization of IL1B (A, C, E) and IL1R1 (B, D, F) in oviductal tissues of S. lanzai (A, B), T. carnifex (C, D), and B. bufo (E, F). Positive staining is shown in red. A) The expression of IL1B is intense and widespread in the luminal epithelial cells, mainly in the basal portion (large arrows) and in the nucleus (asterisk in the insert). Cells of connective tissues (bifurcated arrows) and endothelial cells of the blood vessels (thin arrows) are also highly immunoreactive. The apical portions of the epithelial cells are com- pletely unstained (arrowhead). B) Strong immunoreactivity for IL1R1 in the baso- lateral and perinuclear portions of most luminal epithelial cells (large arrows) and in the cells of the connective tissue (bifurcated arrow). The apical portions of the luminal cells are unstained (arrowhead). C) IL1B immunoreactivity is present in the ciliated epithelial cells (big arrows) and, to some extent, in the cytoplasm of the secretory cells (thin arrow). Secretory granules are unstained. Insert shows intranuclear immu- noreactivity in ciliated epithelial cells (ar- rowhead). D) IL1R1 immunoreactivity is mainly present in the luminal portions of ciliated cells (large arrows). No staining is detected in the nuclei. IL1B (E) and IL1R1 (F) immunoreactivities are confined to the upper portions of the ciliated epithelial cells (large arrows), whereas the glands are completely unstained. Note the immunore- activities for IL1B and IL1R1 in the blood vessel at the base of the ciliated epithelial cells (arrowheads). G, H) Control sections of S. lanzai (G) and T. carnifex (H). No staining is observed when the anti-IL1B or anti- IL1R1 primary antibodies are substituted with Tris-buffered saline.

Article Snippet: Membranes were incubated at 48C overnight with agitation in the presence of goat anti-human IL1B polyclonal antibody (R&D Systems) or goat anti-human IL1R1 polyclonal antibody (R&D Systems) at a dilution of 1:1000 in BS.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Saline

Journal: Biology of reproduction

Article Title: Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.

doi: 10.1095/biolreprod.107.060095

Figure Lengend Snippet: FIG. 3. Western blot profiles. A) IL1B in oviductal tissue lysates from S. lanzai, T. carnifex, and B. bufo. Recombinant human IL1B (rIL1B, positive control). The positions of the molecular weight markers are indicated. Two bands are detected for human rIL1B, corresponding to the predicted mature 17-kDa form and the dimeric 34-kDa form. In S. lanzai, the putative IL1B has a molecular mass of about 20 kDa, whereas in B. bufo and in T. carnifex, the anti-human polyclonal antibody recognizes a protein of about 31 kDa, which is probably pro-IL1B. Note that a band of approximately 12 kDa appears in all three amphibian species. B) IL1R1 in oviductal tissues of S. lanzai, T. carnifex, and B. bufo. Human placenta (Pl) at term is used as a positive control. The position of the molecular weight marker is indicated. A band that corresponds to the 80-kDa human IL1R1 is detected in the human placenta, as well as in S. lanzai and T. carnifex. No band is detected in B. bufo.

Article Snippet: Membranes were incubated at 48C overnight with agitation in the presence of goat anti-human IL1B polyclonal antibody (R&D Systems) or goat anti-human IL1R1 polyclonal antibody (R&D Systems) at a dilution of 1:1000 in BS.

Techniques: Western Blot, Recombinant, Positive Control, Molecular Weight, Marker